• JAWATANKUASA KESELAMATAN DAN KESIHATAN PEKERJAAN


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  • FileName: Safe Laboratory Practice Microbiology Laboratory.pdf [read-online]
    • Abstract: JAWATANKUASA KESELAMATAN DAN KESIHATAN PEKERJAANINSTITUT BIOSAINSUNIVERSITI PUTRA MALAYSIASAFE LABORATORY PRACTICE: MICROBIOLOGY LABORATORYThe following basic safety rules should be observed at all time in the laboratory:

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JAWATANKUASA KESELAMATAN DAN KESIHATAN PEKERJAAN
INSTITUT BIOSAINS
UNIVERSITI PUTRA MALAYSIA
SAFE LABORATORY PRACTICE: MICROBIOLOGY LABORATORY
The following basic safety rules should be observed at all time in the laboratory:
1. Upon entering the laboratory, place coats, books and other paraphernalia in specified locations- never on bench tops.
2. Keep doors and windows closed during the laboratory session to prevent contamination from air currents.
3. At the beginning and termination of each laboratory session, wipe bench tops with a disinfectant solution provided by
the officer.
4. Do not place contaminated instruments such as inoculating loops, needles and pipette tips on bench tops. Loops and
needles should be sterilized by incineration, and pipette tips should be disposed in designated receptacles.
5. On completion of the laboratory session, place all cultures and materials in the disposal area as designated by the
officer.
6. Rapid and efficient manipulation of fungal cultures is required to prevent the dissemination of their reproductive
spores in the laboratory environment.
7. Wash your hands with liquid detergent and dry them with paper towels upon entering and prior to leaving the
laboratory.
8. Wear a paper cap or tie back long hair to minimize its exposure to open flames.
9. Wear a laboratory coat or apron while working in the laboratory to protect clothing from contamination or accidental
discoloration by staining solutions.
10. Closed shoes should be worn at all times in the laboratory setting.
11. Never apply cosmetics or inserts contact lenses in the laboratory.
12. Do not smoke, eat or drink in the laboratory. These activities are absolutely prohibited.
13. Carry cultures in a test tube rack when moving around the laboratory. Likewise, keep cultures in a test tube rack on
the bench tops when not in use. This serves a dual purpose: To prevent accidents and to avoid contamination of
yourself and the environment.
14. Never remove media, equipment or especially bacterial cultures from the laboratory. Doing so is absolutely
prohibited.
15. Immediately cover spilled cultures or broken culture tubes with paper towels and then saturates them with
disinfectant solutions. After 15 minutes of reaction time, remove the towels and dispose of them in a manner
indicated by the officer. (Refer to UPM/TNCPI/S/GP07 Garis Panduan Pengurusan Bahan Sisa Klinikal).
16. Report accidental cuts or burns to the officer immediately.
17. Never pipette by mouth any broth cultures or chemical reagents. Doing so is strictly prohibited. Pipetting is to be
carried out with the aid of a mechanical pipetting device only.
18. Do not lick label. Use only self-stick labels for the identification of experimental cultures.
19. Speak quietly and avoid unnecessary movement around the laboratory to prevent distractions that may cause
accidents.
20. Disposable gloves must be worn during the manipulation of these test materials.
21. Immediate hand washing is required if contact with any of these fluid occurs and also upon removal of gloves.
22. Masks, safety goggles and laboratory coats should be worn if any aerosol might be formed or if splattering of these
fluids is likely to occur.
23. Spilled body fluids should be decontaminated with 1:10 dilution of household bleach, covered with paper toweling,
and allowed to react for 10 minutes before removal.
24. Test specimens and supplies in contact with these fluids must be placed into a container of disinfectant prior to
autoclaving. (Refer to UPM/TNCPI/S/GP07 Garis Panduan Pengurusan Bahan Sisa Klinikal).
Jawatankuasa Keselamatan dan Kesihatan Pekerjaan
Institut Biosains
Universiti Putra Malaysia


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